Elements of Harmony

The Elements of Harmony are a powerful enitity in all of Equestria and featured in the world of Rue Day.

Honesty
"Applejack, who reassured me when I was in doubt, represents the spirit of... honesty!"

- Twilight on Applejack to Princess Luna Honesty is the first known harmony element. Its spirit is represented in Applejack, due to her assuring Twilight that she wouldn't fall and would be caught by Fluttershy and Rainbow Dash.

Kindness
"Fluttershy, who tamed the manticore with her compassion, represents the spirit of... kindness!"

- Twilight

The second element known. Fluttershy represents this when she kindly approaches a manticor because of a thorn in his paw.

Laughter
"Pinkie Pie, who banished fear by giggling in the face of danger, represents the spirit of... laughter!"

- Twilight on Pinkie Pie's element

The third known Element. Pinkie represents this by laughing in the face of danger, mostly at the funny faces in the trees.

Generosity
"Rarity, who calmed a sorrowful serpent with a meaningful gift represents the spirit of... generosity!"

- Twilight on Rarity's element

The fourth element Rarity represents by cutting off her part of her tail and let him use it as a mustache.

Loyalty
"And Rainbow Dash, who could not abandon her friends for her own heart's desire represents the spirit of... loyalty!"

- Twilight on Rainbow's element

Rainbow represents the fifth element. She exhibited this when Princess Luna as Nightmare Moon

Magic
"The spark ignited inside me when I realized that you all... are my friends! You see, Nightmare Moon, when those Elements are ignited by the... the spark, that resides in the heart of us all, it creates the sixth element: the element of... magic"

- Twilight to Nightmare Moon Twilight represents the sixth (believed to be last)  After feeling her friends coming for her, a spark lit in Twilight, thus allowing her to create all five elements again and bear the sixth element.

Fun
Fun is the seventh (and unknown) element of harmony. Its spirit is represented in Rue Day.